Category Archives: 研究發表

蘇昱誠助理教授研究團隊發表研究成果於Communications Chemistry

連結網址：https://www.nature.com/articles/s42004-022-00709-0

Abstract

Covalent attachment of methoxy poly(ethylene) glycol (mPEG) to therapeutic molecules is widely employed to improve their systemic circulation time and therapeutic efficacy. mPEG, however, can induce anti-PEG antibodies that negatively impact drug therapeutic effects. However, the underlying mechanism for specific binding of antibodies to mPEG remains unclear. Here, we determined the first co-crystal structure of the humanized 15-2b anti-mPEG antibody in complex with mPEG, which possesses a deep pocket in the antigen-binding site to accommodate the mPEG polymer. Structural and mutational analyses revealed that mPEG binds to h15-2b via Van der Waals and hydrogen bond interactions, whereas the methoxy group of mPEG is stabilized in a hydrophobic environment between the VH:VL interface. Replacement of the heavy chain hydrophobic V37 residue with a neutral polar serine or threonine residue offers additional hydrogen bond interactions with methoxyl and hydroxyl groups, resulting in cross-reactivity to mPEG and OH-PEG. Our findings provide insights into understanding mPEG-binding specificity and antigenicity of anti-mPEG antibodies.

林奇宏教授研究團隊發表研究成果於 Nature communications
連結:https://www.nature.com/articles/s41467-022-31825-z
Abstract
Regulation of fatty acid uptake, lipid production and storage, and metabolism of lipid droplets (LDs), is closely related to lipid homeostasis, adipocyte hypertrophy and obesity. We report here that stomatin, a major constituent of lipid raft, participates in adipogenesis and adipocyte maturation by modulating related signaling pathways. In adipocyte-like cells, increased stomatin promotes LD growth or enlargements by facilitating LD-LD fusion. It also promotes fatty acid uptake from extracellular environment by recruiting effector molecules, such as FAT/CD36 translocase, to lipid rafts to promote internalization of fatty acids. Stomatin transgenic mice fed with high-fat diet exhibit obesity, insulin resistance and hepatic impairments; however, such phenotypes are not seen in transgenic animals fed with regular diet. Inhibitions of stomatin by gene knockdown or OB-1 inhibit adipogenic differentiation and LD growth through downregulation of PPARγ pathway. Effects of stomatin on PPARγ involves ERK signaling; however, an alternate pathway may also exist.

蕭育源教授研究團隊發表研究成果於 Nature communications

連結: https://www.nature.com/articles/s41467-020-20853-2

Abstract

The exonuclease activity of Apurinic/apyrimidinic endonuclease 1 (APE1) is responsible for processing matched/mismatched terminus in various DNA repair pathways and for removing nucleoside analogs associated with drug resistance. To fill in the gap of structural basis for exonucleolytic cleavage, we determine the APE1-dsDNA complex structures displaying end-binding. As an exonuclease, APE1 does not show base preference but can distinguish dsDNAs with different structural features. Integration with assaying enzyme activity and binding affinity for a variety of substrates reveals for the first time that both endonucleolytic and exonucleolytic cleavage can be understood by an induced space-filling model. Binding dsDNA induces RM (Arg176 and Met269) bridge that defines a long and narrow product pocket for exquisite machinery of substrate selection. Our study paves the way to comprehend end-processing of dsDNA in the cell and the drug resistance relating to APE1.

尤禎祥教授研究團隊發表研究成果Time-resolved Infrared Characterization on the Photolysis of Roussin’s Red Phenyl Ester in Different Solvents於Journal of Photochemistry and Photobiology A: Chemistry

彭慧玲教授研究團隊發表研究成果In-situ monitoring the effect of acoustic vibration in the form of music on the motility of Escherichia coli於Applied Acoustics

廖光文教授研究團隊發表研究成果Secoiridoid Glucosides and Anti-Inflammatory Constituents from the Stem Bark of Fraxinus chinensis於Molecules

黃植懋副教授研究團隊發表研究成果Which digit is larger? Brain responses to number and size interactions in a numerical Stroop task於Psychophysiology

林志生教授研究團隊發表研究成果Bioethanol production from acid pretreated microalgal hydrolysate using microwave-assisted heating wet torrefaction於Fuel

楊進木教授研究成果Membrane protein-regulated networks across human cancers(癌症膜蛋白調節網路)，榮登國際頂級期刊[Nature Communications]