王雲銘教授研究團隊發表研究成果於 Chemical Engineering Journal

連結網址：https://www.sciencedirect.com/science/article/pii/S1385894723001754#f0040

Abstract

Early surveillance of oral cancer demands utmost concern owing to its alarming prevalence in the modern world. An efficient electrochemical impedimetric immunosensor is fabricated based on bimetallic amino-functionalized FeCo metal–organic frameworks uniformly grown on porous nickel foam solid supports (FeCo-MOF/NF) as a transducer for the detection of oral squamous cell carcinoma (OSCC). Herein, the interleukin-1 receptor antagonist (IL-1RA) antibody is used as a biorecognition element for the first time in the determination of oral cancer in real human blood samples using electrochemical impedance spectroscopy (EIS). Furthermore, the presence of specific functional groups ensures selectivity and rapid sensitivity against the target analyte IL-1RA when compared to the other biomarkers including interleukin-6 (IL-6), interleukin-8 (IL-8), CYFRA 21-1, and so on. The immunosensor shows a wide linear dynamic detection range of IL-1RA (10 fg/mL to 10 ng/mL) with a limit of detection (LOD) of 7.30 fg/mL in buffer and 7.22 fg/mL in serum conditions and a limit of quantification (LOQ) of 22.14 fg/mL in PBS and 21.88 fg/mL in serum. For a real-life demonstration, IL-1RA in human samples is detected by the immunosensor for the first time and compared with the gold standard method. The immunosensor also displays an excellent correlation with the standard detection of IL-1RA in human samples. Altogether, this work demonstrates that the electrochemical immunosensor has a high clinical significance by being a promising alternative to conventional approaches.

張家靖教授研究團隊發表研究成果於 Int. J. Mol. Sci.

連結網址：https://www.mdpi.com/1422-0067/24/2/1348

Abstract

Surface contamination by microorganisms such as viruses and bacteria may simultaneously aggravate the biofouling of surfaces and infection of wounds and promote cross-species transmission and the rapid evolution of microbes in emerging diseases. In addition, natural surface structures with unique anti-biofouling properties may be used as guide templates for the development of functional antimicrobial surfaces. Further, these structure-related antimicrobial surfaces can be categorized into microbicidal and anti-biofouling surfaces. This review introduces the recent advances

in the development of microbicidal and anti-biofouling surfaces inspired by natural structures and discusses the related antimicrobial mechanisms, surface topography design, material application, manufacturing techniques, and antimicrobial efficiencies.

趙瑞益教授研究團隊發表研究成果於Pharmaceutics

連結網址：https://www.mdpi.com/1999-4923/15/1/111

Abstract

Abstract: Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy.